WAVE Life Sciences Q2 2026 Earnings Report

WAVE Life Sciences EPS Results

• Actual EPS: -$0.34
• Consensus EPS: -$0.30
• Beat/Miss: Missed by -$0.04
• One Year Ago EPS: N/A

WAVE Life Sciences Revenue Results

• Actual Revenue: $2.27 million
• Expected Revenue: $7.45 million
• Beat/Miss: Missed by -$5.18 million
• YoY Revenue Growth: N/A

WAVE Life Sciences Announcement Details

• Quarter: Q2 2026
• Date: 7/30/2026
• Time: Before Market Opens
• Conference Call Date: Thursday, July 30, 2026
• Conference Call Time: 8:30AM ET

WAVE Life Sciences (NASDAQ:WVE) is a clinical-stage genetic medicines company focused on the discovery and development of stereopure oligonucleotide therapies designed to address serious diseases with high unmet medical need. Leveraging proprietary chemistry and precision synthesis, WAVE engineers drug candidates with defined stereochemistry to optimize potency, safety and manufacturability. This approach aims to enhance target specificity and improve therapeutic profiles compared with traditional oligonucleotide medicines.

The company’s pipeline includes programs in neuromuscular disorders such as Duchenne muscular dystrophy and neurodegenerative conditions including Huntington’s disease, as well as early-stage cardiovascular and liver indications. Several assets are advancing through preclinical research and clinical trials, and WAVE collaborates with biopharmaceutical partners to co-develop selected candidates and expand the reach of its technology into new disease areas.

WAVE’s research and development activities are anchored in its laboratories in Cambridge, Massachusetts, where multidisciplinary teams of chemists, biologists and clinical scientists work to translate novel oligonucleotide designs into development candidates. The company also maintains strategic collaborations and research partnerships in North America and Asia, aiming to accelerate global access to its therapies and support regulatory filings in multiple jurisdictions.

Founded in 2015, WAVE Life Sciences has built a leadership team with deep expertise in RNA biology, medicinal chemistry and drug development. The company continues to refine its stereopure oligonucleotide platform to address delivery challenges, improve pharmacokinetics and expand its pipeline across a broad range of genetic and rare diseases.

WAVE Life Sciences Q2 2026 Earnings Call Transcript

Key Takeaways

• Positive Sentiment: WVE-007 obesity development advanced into Phase IIa with dosing underway in patients with higher BMI and comorbidities. The company is also planning combination-with-incretin and post-incretin maintenance studies this year, targeting fat loss with muscle preservation and potentially once- or twice-yearly dosing.
• Positive Sentiment: WVE-006 produced data the company says support treatment of both lung and liver manifestations of AATD with infrequent subcutaneous dosing. The FDA granted a meeting expected later this summer to discuss a potential accelerated-approval pathway, while data from the 600 mg cohort are expected in the second half of 2026.
• Positive Sentiment: Wave remains on track to submit a CTA for WVE-008 in PNPLA3 liver disease in 2026. The RNA-editing program aims to restore functional protein rather than silence the target, with preclinical data showing more than 50% editing and reduced lipid accumulation versus siRNA.
• Neutral Sentiment: The company is exploring partnerships for its DMD candidate WVE-N531 before filing an NDA, citing an evolving regulatory and commercial landscape. Management said it continues to believe in the program but will reassess filing plans based on regulatory developments and partnership progress.
• Negative Sentiment: Second-quarter revenue fell to $2.3 million from $8.7 million a year earlier, while the net loss widened to $69.4 million from $50.5 million as R&D and G&A spending increased. Wave ended the quarter with $490.6 million in cash and expects funding into Q3 2028, excluding potential future GSK milestone payments.

Presentation

[Operator]

Hello, welcome to Wave Life Sciences' second quarter 2026 earnings call. We ask that you please hold all questions until the completion of the formal remarks. At which time you will be given instructions for the question and answer session. As a reminder, this conference is being recorded today. I will now turn the call over to Kate Rausch, Vice President of Corporate Affairs and Investor Relations.

[Kate Rausch, VP of Corporate Affairs and Investor Relations at Wave Life Sciences]

Thank you, operator, good morning to everyone on the call. Earlier this morning, we issued a press release outlining our second quarter 2026 earnings update. Joining me today with prepared remarks are Dr. Paul Bolno, President and Chief Executive Officer, Dr. Erik Ingelsson, Chief Scientific Officer, Dr. Chris Wright, Chief Medical Officer, and Kyle Moran, Chief Financial Officer. The press release issued this morning is available on the investor section of our website, www.wavelifesciences.com.

[Kate Rausch, VP of Corporate Affairs and Investor Relations at Wave Life Sciences]

Before we begin, I would like to remind you that discussions during this conference call will include forward-looking statements. These statements are subject to several risks and uncertainties that could cause our actual results to differ materially from those described in these forward-looking statements. The factors that could cause actual results to differ are discussed in the press release issued today and in our SEC filings.

[Kate Rausch, VP of Corporate Affairs and Investor Relations at Wave Life Sciences]

We undertake no obligation to update or revise any forward-looking statement for any reason. I would now like to turn the call over to Paul.

[Paul Bolno, President and CEO at Wave Life Sciences]

Thanks, Kate, good morning to everyone joining us on today's call. At Wave, we are focused on harnessing the convergence of deep genetic insights with our proprietary chemistry to rapidly advance a pipeline of transformational RNA medicines. Over the past decade, we have built the most sophisticated and broadly enabled oligonucleotide platform in the industry. Multiple clinical data sets now demonstrate our ability to rapidly advance programs from novel genetic target to proof of mechanism in the clinic.

[Paul Bolno, President and CEO at Wave Life Sciences]

Our proprietary chemistry is what differentiates our pipeline and distinguishes our molecules from others in the field. In the first half of 2026, we delivered multiple positive data sets across our RNAi and RNA editing pipeline, led by WVE-007 for obesity and WVE-006 for AATD, positioning us to advance both programs to their next stage of development.

[Paul Bolno, President and CEO at Wave Life Sciences]

We also continue to progress our second RNA editing candidate, WVE-008, for PNPLA3 liver disease, remain on track for a CTA filing later this year. In March, we shared positive data from the single-dose phase I portion of the INLIGHT trial of WVE-007 in obesity. These data fortified our conviction in a best-in-class siRNA design with robust and extremely durable silencing demonstrated in the clinic, supporting potential dosing of once or twice a year.

[Paul Bolno, President and CEO at Wave Life Sciences]

Even in this otherwise healthy population, a single dose of WVE-007 led to substantial reductions in visceral fat and subcutaneous fat without muscle loss or other adverse events associated with incretin therapy. We have three opportunities to explore in the next phase of development for this program.

[Paul Bolno, President and CEO at Wave Life Sciences]

First, evaluate WVE-007 in a phase II-A population with higher BMI and comorbidities typical of other obesity trials to demonstrate WVE-007's potential in obesity, as well as evaluate biomarkers to unlock other cardiometabolic indications, including MASH and type 2 diabetes. Second, evaluate combination with incretin, which has the potential to deepen weight loss and enhance metabolic benefits. Third, perhaps one of the most exciting and unique opportunities, evaluate maintenance therapy, which would represent an entirely new commercial frontier enabling patients an off-ramp for their GLP-1s.

[Paul Bolno, President and CEO at Wave Life Sciences]

We often hear from patients about the fear of weight regain post-cessation of incretins. Up to 70% of patients discontinue GLP-1 therapy within the first year of treatment, for many, it's due to challenges that WVE-007 may address, including tolerability issues, treatment burden, or anhedonia, loss of joy, among others.

[Paul Bolno, President and CEO at Wave Life Sciences]

In this maintenance setting, WVE-007 would have the potential to enable individuals and payers to realize the sustained health benefits of fat loss and improve body composition for the long term. Over the past quarter, the FDA accepted the phase II-A INLIGHT trial amendment, dosing is now underway. The phase II-A is enrolling individuals living with obesity with BMIs between 35 and 50, and comorbidities with or without diabetes.

[Paul Bolno, President and CEO at Wave Life Sciences]

These patients are expected to have higher total body fat and higher levels of visceral fat than the phase I otherwise healthy patients. With this multi-dose clinical trial, we are evaluating our target patient population and have the potential to deliver further improvements in body composition, meaning weight loss driven off of fat loss without muscle loss, as well as other improvements in cardiometabolic biomarkers, including liver fat and HbA1c.

[Paul Bolno, President and CEO at Wave Life Sciences]

We are also working expeditiously to initiate the additional phase II trials of WVE-007 in combination and maintenance settings this year. Collectively, our development program has the potential to redefine the treatment landscape in obesity. In RNA editing, we delivered data supporting 006's potential to offer a new standard of care for individuals living with AATD by treating both lung and liver manifestations of the disease and restoring the dynamic AAT protein response with convenient, infrequent subcutaneous dosing.

[Paul Bolno, President and CEO at Wave Life Sciences]

We are planning to engage the FDA as the agency has granted our request for a meeting to discuss a potential accelerated approval pathway for WVE-006. This meeting is expected at the end of summer and will help inform our registrational plans and path toward bringing a much-needed new treatment option to the 200,000 individuals in the U.S. and Europe living with homozygous ZZ AATD.

[Paul Bolno, President and CEO at Wave Life Sciences]

As a scalable, infrequent subcutaneously dosed oligonucleotide therapy, 006 would offer a potentially differentiated value proposition for both healthcare providers and payers. Current IV augmentation standard of care does not have any impact on liver manifestations of the disease, while investigational DNA editing, in addition to any safety concerns, would be associated with payer challenges for one-time costly therapy with uncertain durability and multi-year enrollee retention.

[Paul Bolno, President and CEO at Wave Life Sciences]

Building on our RNA editing success, we are advancing our second RNA editing candidate, WVE-008, toward the clinic this year for PNPLA3 liver disease. 008 aims to address an area of high unmet need, with 9 million individuals living with homozygous PNPLA3 I148M liver disease. Human genetic data demonstrates these homozygous individuals have about a nine-fold higher risk of dying from liver disease as compared with non-carriers.

[Paul Bolno, President and CEO at Wave Life Sciences]

The landscape of therapies and development for this target has been narrowed by recent clinical demonstration that silencing approaches do not address the disease and may actually exacerbate it. An RNA editing approach is the only way to restore the functional protein and offers a potential for a novel, infrequently dosed therapy with strong support from human genetics. Beyond our lead RNAi and RNA editing programs, we continue to push the boundaries of innovation with our proprietary chemistry.

[Paul Bolno, President and CEO at Wave Life Sciences]

We are planning to host our annual investor day in the fall to shed more light on our latest platform advancements and work on our bifunctional modality. At the start of the year, we outlined our pipeline priorities, WVE-007, 006, and 008, demonstrating our core focus on RNAi and RNA editing, as well as our intent to seek partnership opportunities for HD and DMD.

[Paul Bolno, President and CEO at Wave Life Sciences]

Given the evolving regulatory and commercial landscape in DMD, we are exploring potential partnerships in advance of filing an NDA for WVE-N531. We continue to believe in WVE-N531 and WVE-003's potential as best-in-class treatment options and look forward to continuing our partnering discussions.

[Paul Bolno, President and CEO at Wave Life Sciences]

With our continued success in the clinic and robust balance sheet, we are well-positioned and well-capitalized to bring our pipeline of potential first and best-in-class candidates to the next stage of development as we reimagine what's possible for patients. Now I'd like to turn the call over to Erik, who will discuss WVE-007 and how we are leveraging our proprietary chemistry and human genetic insights to advance a transformative approach for obesity and other cardiometabolic diseases. Erik?

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Thank you, Paul. I'll start today by discussing our Inhibin E GalNAc siRNA, WVE-007, and how our program aims to fill a large unmet need with a differentiated approach grounded in human genetics. Today, there are over 1 billion individuals living with obesity globally, including 175 million in the U.S. and Europe alone.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

These individuals face markedly higher risks of a range of diseases, such as MASH, type 2 diabetes, and other cardiometabolic diseases. Excess body fat, in particular visceral fat, is the key driver behind this elevated risk of disease. While there are several therapeutic options available for weight loss and countless more in clinical development with similar modes of action to those already on the market, these therapies come with several limitations.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Current standard of care therapies reduce body weight through both fat loss and muscle loss. They carry high discontinuation rates due to the GI side effects, limiting potential for long-term health benefits. The loss of muscle associated with current weight loss therapies is substantial, with up to 40% of the total weight loss.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

This has significant health implications, as skeletal muscle, in addition to muscle strength and function, also plays a key role in metabolism by sustaining basal metabolic rate, glucose disposal, and insulin sensitivity. Muscle also prevents weight regain, which occurs mostly from fat in a majority of individuals that discontinue incretin therapies. Remember, as much as up to 70% of individuals discontinue incretins within the first year of treatment.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

An ideal obesity therapy should instead selectively reduce excess fat, including harmful visceral fat, the fat surrounding one's organs that is most strongly linked to MASH, type 2 diabetes, and other cardiometabolic diseases, while also lowering subcutaneous fat and liver steatosis, and critically preserves skeletal muscle. It is well established that a 5%-10% reduction in visceral fat mass is associated with positive health outcomes by reducing risk of multiple preventable metabolic diseases and preserving patient function and quality of life.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

All these benefits can be delivered by 007's mechanism of action. Rather than acting on appetite, 007 silences Inhibin E and lowers serum Activin A, a liver-derived peptide that signals adipocytes to put the brakes on lipolysis. Removing those brakes drives fat loss without calorie restriction and without the muscle loss seen with incretin-based therapies.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Our Inhibin E approach is strongly grounded in human genetics, as carriers of heterozygous Inhibin E loss-of-function variants, nature's own knockdown experiment, exhibit a healthier overall metabolic profile. As Paul discussed earlier, 007's unique ability to durably suppress Inhibin E is driven by our proprietary chemistry and SpiNA siRNA design.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Our next-generation SpiNA designs enhance interactions with AGO2, stabilize the loaded RISC complex, and improve liver exposure, all of which contribute to dramatically improved silencing potency and durability when compared with industry-leading siRNA designs, something we have shown repeatedly for Inhibin E and other targets. While RNAi is a well-established therapeutic modality and there are extensive human genetics data supporting Inhibin E as a target, we believe our proprietary chemistry distinguishes us from others attempting a similar approach.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Our interim phase I INLIGHT data sets from lower BMI, otherwise healthy individuals, confirm that this proprietary chemistry and the underlying human genetics are already translating in the clinic. We have observed consistent, durable, and dose-dependent serum activity reductions of up to 88%, which were sustained through at least seven and a half months, supporting 007's potential for once or twice-yearly dosing.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Notably, we observed the translation of target engagement to substantial improvements of cardiometabolic risk factors, with preservation of lean mass and clinically meaningful reductions in total fat, visceral fat, and waist circumference after just a single dose. To provide context for our results at this early development stage, we calculated the Visceral Fat to Muscle Ratio, or VMR, which is a measure of body composition that integrates harmful visceral fat and beneficial lean mass into a single index.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Lower VMR is associated with a decreased risk of MASH, type 2 diabetes, and cardiometabolic disorders. With a single dose of 007 in our phase I population, we already observed a 16.5% improvement in VMR, which was more than 12.2% achieved with weekly semaglutide in the phase II BELIEVE study and approached the 18.8% observed with the bimagrumab.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

We believe VMR has the potential to serve as a novel composite biomarker that captures body composition improvements more holistically than BMI, that may better predict long-term clinical benefits. Together with the patient community and KOLs, we're working to engage with regulators on the importance of improving body composition, including decreasing excess fat and preserving muscle consistent with their recent guidance.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

What makes the comparison with the BELIEVE study particularly exciting is that our INLIGHT participants had substantially lower baseline BMI, lower visceral fat, and lower total fat compared to phase II and phase III obesity studies, including the BELIEVE study. Clinical experience also highlights the importance of baseline adiposity. Early phase I studies in leaner individuals typically show more modest fat reductions, while studies of individuals with higher baseline obesity demonstrate substantially larger decreases in total and visceral fat mass.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Early follow-up from our 400 mg cohort, which included a substantially higher proportion of individuals with low levels of body fat, also confirmed that higher baseline visceral fat led to greater visceral fat reductions overall. As Chris will speak to shortly, we've been working expeditiously to advance 007 into participants with higher BMI and comorbidities in the phase IIa portion in INLIGHT, where existing science predicts a larger effect.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Activin binds ALK7 on all adipocytes, and visceral fat, being more metabolically active and better perfused, mobilizes first, exactly what we have observed in phase I. With more excess fat to lose, we expect both visceral and total fat loss with 007 to be substantially more pronounced in higher BMI participants in the phase IIa study. For further detail on our 007 development plans and our RNA editing programs, I'd now like to turn the call over to Chris.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

Thanks, Erik. As you recently announced, we're excited to have advanced WVE-007 to a population well-suited to its mechanism of action. We would expect even more pronounced effects on visceral and subcutaneous fat loss. Dosing is currently underway in the phase IIa multi-dose portion of INLIGHT.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

This global placebo-controlled trial will enroll individuals with higher BMIs in the range of 35-50 and comorbidities across 2 dose levels, 240 mg and 400 mg, and 2 study populations with and without type 2 diabetes, for a total of 4 cohorts of 40 patients each. Assessments in this multi-dose portion are like those in the single-dose portion, with additional inclusion of body composition measured by MRI, liver fat content as measured by MRI-PDFF, HbA1c, lipid levels, and other measures.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

The design and study population enables enhanced evaluation, not only of improved body composition and weight loss, but also informs additional opportunities for WVE-007 in MASH, type 2 diabetes, and cardiometabolic diseases. Participants will be given two doses of WVE-007, one at day 1 and one at day 85, and followed for 12 months, with the first key assessments occurring at day 85.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

We believe that WVE-007's orthogonal mechanism, ability to drive fat reductions while preserving muscle, and favorable safety profile are also aptly suited to combination and maintenance approaches. Our preclinical data provides compelling support for both use cases. We've observed approximately twofold greater weight loss as an add-on to incretin versus incretin alone in obese mice and have demonstrated the ability to curtail weight regain following cessation of incretin.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

Planning is well underway for clinical studies addressing incretin combination and post-incretin maintenance and remain on track to initiate this year. We also expect to share additional data from the phase I portion of INLIGHT this year, including data from our 600 milligram cohort, which will further inform the durability of WVE-007. Turning to our ongoing RestorAATion-2 clinical trial of WVE-006 for AATD. AATD is a uniquely compelling disease for RNA editing.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

It is a monogenic disorder caused by a single well-characterized genetic variant in the SERPINA1 gene. This leads to misfolded Z-AAT protein and an absence of healthy circulating M-AAT protein, which normally protects the lung during inflammation or infectious events. Without dynamic production of functional AAT protein, individuals with alpha-1 are at risk for lung damage and ultimately developing emphysema and bronchiectasis, which is characterized by chronic cough, recurrent infections, and shortness of breath.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

In parallel, misfolded Z-AAT accumulates in hepatocytes and causes progressive liver injury and increased risk of liver disease. Approximately 200,000 individuals in the U.S. and Europe live with homozygous PIZZ AATD. Currently, the only approved treatment for AATD is weekly IV plasma-derived augmentation therapy, which carries several limitations. With a fixed schedule dose, there's no restoration of dynamic response, leaving individuals with alpha-1 at risk if AAT protein levels fall too low during an infectious or inflammatory event.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

IV therapy is time-consuming and often requires in-patient visits and does nothing to lower Z-AAT to address the risk of liver disease. Investigational therapies and development also come with several limitations. DNA-based editing approaches target both the lung and liver, but they introduce permanent DNA modifications, carry bystander editing risk, and rely on LNP delivery, which is associated with liver enzyme elevations.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

The risk of irreversible off-target effects is particularly notable, as genomic DNA editing has been connected to editing in cancer-associated genes. Also in development are AAT siRNA approaches, which reduce Z-AAT. However, they do not restore M-AAT, potentially exacerbating lung disease through chronic AAT knockdown. WVE-006 has the potential to be the first treatment for AATD that enables individuals with alpha-1 to produce protective AAT protein when needed most and address the root cause of the disease with a convenient and infrequent subcutaneously dosed therapy.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

With WVE-006, our goal is to recapitulate an MZ-like phenotype, as it's well established that heterozygous individuals have low risk of both lung and liver disease.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

MZ individuals, compared to ZZs, have reduced levels of Z-AAT, which protects the liver from damage and are able to protect the lung with basal AAT levels above 11 micromolar, of which at least 50% is wild type M-AAT, and most importantly, are able to mount a dynamic AAT response during an acute infection. That combination, Z-AAT reduction, protective basal levels with a meaningful proportion of wild type M-AAT, and a preserved acute phase response, is the bar we set for WVE-006.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

As we shared in May, this is exactly the profile we've consistently achieved. WVE-006 delivered a compelling therapeutic profile following only three months of treatment across both 200 milligram biweekly and 400 milligram monthly dosing. Importantly, the WVE-006 RNA editing approach produces only wild type canonical M-AAT and does not include bystander edited isoforms, as seen with DNA editing.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

This specificity is crucial, as bystander edits not only introduce functional activity risk, but may also render patients ineligible for future base editing or RNA editing therapies. Enrollment and dosing are now complete in the 200 milligram, 400 milligram, and 600 milligram cohorts of RestorAATion-2. We remain on track to share data from the 600 milligram monthly dosing cohort in the second half of this year, which will help inform an optimal dose regimen.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

With the compelling profile of WVE-006 we've observed to date, we're continuing to engage with the AATD community, key opinion leaders, and advance our discussions with regulators. We're excited to announce today that the FDA granted our request for a meeting, which is planned for the end of this summer. During this, we intend to discuss a potential accelerated approval pathway for WVE-006 and AATD.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

Feedback from this meeting will help inform our potential registration study design and our plans to efficiently advance WVE-006 for ZZ individuals with alpha-1 who are in urgent need of new treatment options. Building on our success with WVE-006, we are advancing our second RNA editing clinical candidate, WVE-008, for homozygous PNPLA3 I148M liver disease. Similar to WVE-006 and WVE-007, our approach to WVE-008 is deeply grounded in genetics. The PNPLA3 variant is a well-established driver of NASH and liver diseases more generally.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

Yet, there are no approved medicines that directly address this biology. There are an estimated 9 million homozygous PNPLA3 I148M carriers across the U.S. and Europe who are at a nine-fold higher risk of dying from their liver disease compared to non-carriers. Currently, the only treatment options are non-precision medicines aimed at reducing liver steatosis and early fibrosis with limited efficacy for these I148M carriers.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

Silencing PNPLA3 can only partially address disease biology. It's likely to leave residual pathology, since it knocks down all PNPLA3 protein without restoring healthy wild type protein, which has important physiologic functions in the liver. As a result, silencing partially addresses steatosis, but inflammation and fibrosis remain unaddressed, or worse.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

Recent clinical trials of PNPLA3 silencing support this notion, as dose-dependent increases in liver enzymes were observed. By contrast, with WVE-008, we aim to correct the I148M variant using our leading RNA editing capability, which is expected to restore PNPLA3 activity and lipid mobilization, reversing steatosis and fibrosis and improving liver health. In May, we shared pre-clinical data supporting our approach at EASL, the European Association for the Study of the Liver Congress.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

We demonstrated that our PNPLA3 AIMers delivered substantial editing of the I148M transcript, exceeding the 50% threshold expected to lower risk for liver disease, achieved concentrations in the liver expected to support substantial editing and decrease lipid droplet density more than siRNA.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

In our upcoming first-in-human study of 008, we plan to leverage previously genotyped populations to efficiently identify homozygous I148M carriers and accelerate enrollment. We will evaluate target engagement with circulating biomarkers and assess early signs of efficacy using non-invasive imaging. We remain on track for a CTA submission in 2026. With that, I'll turn the call over to Kyle to provide an update on our financials. Kyle?

[Kyle Moran, CFO at Wave Life Sciences]

Thanks, Chris. Our revenue for the second quarter of 2026 was $2.3 million, compared to $8.7 million in the prior year quarter, and relates to our ongoing collaboration agreement with GSK. Research and development expenses were $51.3 million in the second quarter of 2026 as compared to $43.5 million in the same period in 2025.

[Kyle Moran, CFO at Wave Life Sciences]

The increase primarily reflects continued investment in advancing our clinical programs, including preparation for the phase IIa portion of INLIGHT and continued progress on our RNA editing pipeline. Our G&A expenses were $24.8 million for the second quarter of 2026 as compared to $18 million for the prior year quarter. The increase primarily reflects costs associated with supporting our expanding pipeline and preparing for the next stages of development.

[Kyle Moran, CFO at Wave Life Sciences]

As a result, our net loss was $69.4 million for the second quarter of 2026 as compared to a net loss of $50.5 million in the prior year quarter. We ended the second quarter with $490.6 million in cash equivalents, and marketable securities, which we expect to be sufficient to fund operations into Q3 2028. While we expect to receive milestone payments from GSK in the second half of 2026, it's important to note that potential future milestones and other payments to us under our collaboration are not included in our cash runway. I'll now turn the call back over to Paul for closing remarks.

[Paul Bolno, President and CEO at Wave Life Sciences]

Thank you, Kyle. As we look to the second half of 2026, we believe we are well-positioned to unlock value across our pipeline. With WVE-007, our phase IIa trial in individuals with obesity is underway, and we are rapidly working to advance incretin combination and post-incretin maintenance studies later this year.

[Paul Bolno, President and CEO at Wave Life Sciences]

With 006, we are on track to meet with regulators on a pathway to accelerated approval, and we are also working to bring our second RNA editing candidate into clinical development for PNPLA3 liver disease in the second half of this year. Before turning to questions, I want to thank our team for the progress we've made and for their continued commitment to reimagining what's possible for patients. I'll now turn the call over to the operator. Operator?

[Operator]

We will now move into our Q&A session. For those of you who are joining us by the Q&A webcast, if you would like to ask a question at this time, please raise your hand by clicking the raise your hand at the bottom of your window. We would like to ask all analysts to please limit yourself to one question per person. Once called upon, please unmute your audio to ask a question. We will take our first question from Yun Zhong with Wedbush. Please unmute your line and ask your question.

[Yun Zhong, Analyst at Wedbush]

Hi. Good morning. Thank you very much for taking the questions. The question is on the WVE-007 program for obesity. I wanted to confirm that for the ongoing phase IIa portion, the goal is to confirm that you are able to achieve 5% weight loss as compared to placebo at 12 months to support the advancement for this program into the indication of obesity.

[Yun Zhong, Analyst at Wedbush]

On combination therapy, I believe other programs have reported or will report data. Given the same mechanism of action, what would be your expectation on data from your own program? Do you think you will potentially be able to show any differentiation? Thank you very much.

[Paul Bolno, President and CEO at Wave Life Sciences]

Thank you. I'll start with the second question, then we'll work backwards to the first question, because I think the answer to the second question is, we do believe that it's valuable to see what others have been generating in that space, not just for the potential for increasing fat loss. Remember, these other datasets that people have seen in combination were in phase IIa, high BMI, high visceral fat, high total fat patient populations. They were able to see, as you point out, substantial reductions in both fat, visceral fat in particular, and liver fat. We should remind that the liver fat was pretty substantial.

[Paul Bolno, President and CEO at Wave Life Sciences]

Given that in comparison, both on preclinical ED50, where we're about threefold more potent and in our preclinical data where we've seen increased potency, we would expect to see those data as affirming and that we would continue to expect to see with greater, more durable knockdown, potentially better data.

[Paul Bolno, President and CEO at Wave Life Sciences]

I think it's just great to see that the field sees that in the combination setting. We're also excited about maintenance because I think where we've generated data there and where the human genetics lie, maintenance is an incredibly interesting opportunity for us, in differentiation. Getting back to your first question, which I think is critical for people to hear, the phase IIa is designed, as you point out, to be able to elicit that regulatory and cross that regulatory threshold of 5%.

[Paul Bolno, President and CEO at Wave Life Sciences]

I think as we've been pretty consistent with looking at other studies like BELIEVE with bimagrumab, where there was less fat loss in this healthy population than we saw. Actually, that medicine had a greater lean mass gain than what we have. If we think about that equation and put our medicine into this population, We should see weight loss. bimagrumab had greater than 5% weight loss in that treatment setting. I think it's also important to point out that the phase IIa design is more consequential than just looking at and hitting that target.

[Paul Bolno, President and CEO at Wave Life Sciences]

Remember, we built into the study intentionally by looking at diabetics and non-diabetics, adding MRI-PDFF, the opportunity to expand this more broadly in cardiometabolic disease beyond just the obesity threshold in terms of looking at the treatment of MASH, where again, we look at the comparators in this population.

[Paul Bolno, President and CEO at Wave Life Sciences]

That ability to see substantial liver fat reduction is consequential in the field of MASH, we'd want to see that data. We'd want to see the data on HbA1c, and we'd also want to see the data on lipids. I think in the totality of this as a cardiometabolic drug, including obesity, I think this study is designed and poised to unlock that opportunity.

[Operator]

We'll take our next question from Samantha Semenkow with Citi. Please unmute your line and ask a question.

[Ben Asuncion, Analyst at Citi]

Hi, this is Ben on for Sam. Thanks so much for taking the question. I guess maybe following up on the first question, in the phase IIa INLIGHT trial, what metrics are you focused on and what magnitude of improvement do you need to see to support specific expansion into MASH or type 2 diabetes or the other cardiometabolic diseases? Thanks.

[Paul Bolno, President and CEO at Wave Life Sciences]

Thank you. I'll let Chris talk about the endpoints that we're looking at and then can affirm, Chris, you want to talk about the field what we're evaluating?

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

We're looking at MRI-PDFF, looking at liver fat in the phase IIa study. I know that there are other compounds in the same mechanism in the same area that have shown substantial decreases in liver fat, that really are highly competitive with what's out there in the MASH space. We'll also be looking at hemoglobin A1c.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

We are looking at both non-diabetics and diabetics, and that's a measure that can change over a relatively short time frame and can be utilized as a registration endpoint. We're excited to see what types of benefits we would have there. Lastly, we'll be looking at a number of the lipid profiles to understand if there's a potential there as well to move forward into a lipid registration type study, since there's very tight connections between lipid levels and cardiovascular outcomes.

[Paul Bolno, President and CEO at Wave Life Sciences]

Just to add on, that's in connection additionally with the other measurements. Being able to look at both DEXA and MRI, which will give us that opportunity to assess visceral fat volume and total fat. That's important because I know a lot of people want to always step back and put these datasets of our phase I up against other phase II, III datasets and create tables, and I appreciate the need for folks to do that.

[Paul Bolno, President and CEO at Wave Life Sciences]

I think what is important is to remember in that low fat, low BMI, low visceral fat population, that even when we do compare that against comparator studies where people have said, "Oh, your visceral fat reductions look relatively similar." Our visceral fat compartment was one liter versus five liters, almost five times as much visceral fat where we were seeing that reduction.

[Paul Bolno, President and CEO at Wave Life Sciences]

The ability that we've seen in this phase I, otherwise healthy population, is highly encouraging. We do want to be able to, for that comparator work, have MRI imaging to be able to set the comparators up where we have a high-resolution imaging system to be able to do that. In addition, we'll look at other measurements like grip strength and others and be able to look at muscle retention.

[Paul Bolno, President and CEO at Wave Life Sciences]

I do think when we do step back and think about this therapy in the context from a particular payer perspective and a patient perspective, yes, we want the cardiometabolic health benefits associated with diabetes, but this muscle retention, as Erik pointed out, is critically important. I think we oftentimes think about muscle as strength. We can be able to assess that, but muscle is also a metabolic organ.

[Paul Bolno, President and CEO at Wave Life Sciences]

The ability to look at that ability to retain and sustain muscle in the setting of that fat reduction are all endpoints we'll look at as well, like weight circumference and total body weight. Again, there's a number of endpoints that we'll be assessing as part of this study broadly that encompass both obesity and cardiometabolic indications in totality.

[Operator]

Our next question comes from Steve Seedhouse with Cantor Fitzgerald. Please unmute your line and ask a question.

[Nick Econom, Analyst at Cantor Fitzgerald]

Hi, thank you for the question. This is Nick on for Steve. We just wanted to clarify, in the FDA meeting end of summer, have you already received some feedback on an accelerated approval pathway and subsequently requested a meeting to discuss? Or do you not yet have any feedback yet on a proposed accelerated pathway and this meeting in the end of summer will constitute the first feedback from the FDA?

[Paul Bolno, President and CEO at Wave Life Sciences]

Thank you for the question. This would be the first meeting with the FDA. When we submitted for the meeting, they granted that, and this is when it's scheduled. We would expect after that meeting to provide an update after we have written feedback. We do appreciate the clarifying question that this is the first request for a meeting, and we're excited that instead of a written response, the FDA granted a face-to-face discussion on an accelerated approval pathway.

[Operator]

Our next question comes from Joseph Schwartz with Leerink Partners. Please unmute your line and ask your question.

[Joseph Schwartz, Analyst at Leerink Partners]

Great. Thanks for the update. I was wondering if you could give us some more insight into how enrollment is progressing in the higher BMI phase IIa cohorts for WVE-007 and what you're seeing in terms of screen failure rates and baseline characteristics, and when investors should expect the first meaningful data from these cohorts. Thanks.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

We're currently making really great progress on enrollment. We're in the process of opening additional sites and, once we have everything up and running well, we'll be able to comment more on the timing. So far, there's been really great interest in the study, and it's enrolling extremely well.

[Paul Bolno, President and CEO at Wave Life Sciences]

Yeah. Just to echo Chris' sentiment, momentum coming off the last dataset, Ty, both ex-U.S. but also importantly on this study, U.S. As we think about this study having actually the expansion, we're excited to expand the number of sites and accelerate it. Also to Chris' point, remember, this study has three-month time points and other assessments. We'll continue to look at the rate of enrollment to guide. While the potential is there for 2026, we have to see how we're progressing in this study ultimately to provide that guidance.

[Operator]

Our next question comes from Whitney Ijem with Canaccord Genuity. Please unmute your line and ask your question.

[Angela Qian, Analyst at Canaccord Genuity]

Hey, guys. This is Angela on for Whitney. Thank you for taking our question. Maybe just another one on 007. Looking at the human genetic data, Inhibin E loss of function does demonstrate the improved fat distribution as you've discussed, but not necessarily correlated with BMI as we understand it. What gives you confidence that we'll be able to see BMI improvement with monotherapy in the phase IIa?

[Paul Bolno, President and CEO at Wave Life Sciences]

I think the correlation. It's interesting, and I'll move back and forth with Erik on this one. I think what is interesting when we go back, there was a poster that we shared, and actually Alnylam had done the work on a large, I think it was for 300 patients, as kind of a natural history cohort in non-diabetics.

[Paul Bolno, President and CEO at Wave Life Sciences]

Actually showed Activin E, if we think about the actual ligand itself, actually had strong correlation with BMI, insulin sensitivity, and truncal fat. The data and correlation between the ligand and BMI is well correlated. I think the challenge in trying to, and I think you bring up a very good point on the assessment of why BMI in itself is not a great indicator of body composition is, you could be a bodybuilder and have higher BMI.

[Paul Bolno, President and CEO at Wave Life Sciences]

I think the context of really thinking about the addition of comorbidities where you get higher subcutaneous fat, higher visceral fat, in addition to that becomes an important criteria and characteristic as we think about improvements in body composition. I think what's encouraging is, again, even in the setting in the phase I otherwise healthy patients, we did see substantial reductions in subcutaneous fat, visceral fat, saw 3.3% reduction of waist circumference.

[Paul Bolno, President and CEO at Wave Life Sciences]

I do think the ability to see those changes, even in the lower BMI setting, sets us up for encouraging changes when we move to high BMI. Again, with comorbidity, those patients, when you look at their characteristics and disposition, are also high subcu fat, high visceral fat, and obviously that's mechanistically what we've seen in reduction in the animal models.

[Paul Bolno, President and CEO at Wave Life Sciences]

Erik, I don't know if there's anything, or if you want to just add additional comments to that.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

I think you covered most of it, but maybe just to add a few things. The phenotype in the genetic studies were adjusted for BMI. Just the design when we set that up now 20 years ago, those type of phenotypes that look at body composition, we wanted to adjust out an overall body size. Just by design, it won't be associated with BMI.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

That's the first thing. The second thing is that human genetic carriers, they have 50% reduction, and we think we need to get to over 70% based on what we see in our animal data where we definitely do see total weight loss. I guess the last thing is that we already see total body fat reduction with one single dose, the 240 milligram cohort.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

We're pretty confident about that now as we're rolling into higher BMI with much more excess fat to lose and multiple doses, that we will get to the real weight loss as well. Just based on the total fat loss that will accumulate in the phase II.

[Operator]

Our next question comes from Salim Syed with Mizuho. Please unmute your line and ask your question.

[Salim Syed, Analyst at Mizuho]

Hey, guys. Thanks for all the color today. Paul, maybe just one from us on the DMD side. Can you just provide a little bit more color exactly what caused the shift here to, I guess, now evaluate potential partnerships in advance of filing the NDA for 531? If you don't get the partnership, is there any scenario here where you still plan to file the NDA? Thanks so much.

[Paul Bolno, President and CEO at Wave Life Sciences]

No, thank you. I think we've been clear at the start of this year that we were going to seek a partnership for N531. I think we remain consistent in discussions on partnering N531. I think to the point of, the last part of the question of would we file absent the partner in advance, file in advance of a partnership, I think we're going to continue to evaluate the space. I think what's evolved in this space, particularly with the update that the existing PMO-53 golodirsen is going to file for potential full approval early in the year. There'll be regulatory feedback around that.

[Paul Bolno, President and CEO at Wave Life Sciences]

I think in advance of recognizing that you need to fully enroll or have well underway a confirmatory study in advance wouldn't be prudent at this point in time until we understand that pathway. We continue to have the discussions that are ongoing around potential partnerships and collaborations. I think we've just evaluated all of that relative to where the current funds are invested. That was the decision we made.

[Operator]

Our next question comes from Alex Stranahan with Bank of America. Please unmute your line and ask your question.

[Alex Stranahan, Analyst at Bank of America]

Hey, guys. Thanks for taking our questions. Maybe one for Paul. Curious if, after regaining the rights to 006, whether the pace or the nature of the FDA interactions have changed within AATD. I guess looking ahead to the meeting this summer, is your expectation in line with endpoints and study size based on recent precedents or any sort of unique aspects of your data set that could warrant, say, an even smaller study, for example? Thank you.

[Paul Bolno, President and CEO at Wave Life Sciences]

No, thank you for the question. I think the short answer to your first part of your question is yes. Obviously, by regaining the rights means that we can control the pace of regulatory interactions, and with that could obviously accelerate them. That's obviously wonderful.

[Paul Bolno, President and CEO at Wave Life Sciences]

I think to the second point, we wouldn't expect differences in terms of biomarker-driven approaches for potential pathway, but I think it's again incumbent upon us, and which is why we want to have a face-to-face meeting to get alignment, not just on that biomarker-driven pathway to potential accelerated approval, but as you point out, just because others have requirements in terms of duration of study and numbers of patients may not be equivalent for us. If we're doing a repeat administration, we may not have to run as long a period of time.

[Paul Bolno, President and CEO at Wave Life Sciences]

One of the other nature of the conversation that we're interested in is being able to open and explore what the full approval pathway looks like and how we might be able to more efficiently design a study that could have an interim analysis for the biomarker-driven accelerated approval with the potential to sustain those patients on for full approval, so we expedite the time and pathway to get there. All of this will be the nature of the discussions with the agency, and we're excited to have that.

[Operator]

Our next question comes from Madison El-Saadi with B. Riley. Please unmute your line and ask your question.

[Madison El-Saadi, Analyst at B. Riley]

Hey, guys. Good morning, and thank you for taking our question. What MRI-PDFF liver fat reduction would the team consider a meaningful threshold to MASH? Is this something we would benchmark it against Rezdiffra, which I think was around a 30% relevant reduction? Is there a population difference we should be aware of? Maybe if you could just touch on your fall investor day, if this is something that's really anchored by WVE-006 regulatory update, obesity progress, or is this really more about the broader, earlier stage platform? Thanks.

[Paul Bolno, President and CEO at Wave Life Sciences]

Thanks, Madison. I think at a high level, look, as you point out, we've already seen from others in the field, in a high BMI, high fat setting, that they could achieve 44% reduction, greater than existing therapies and products that are approved in MASH. I think the potential's out there. I think with signals, I think we're going to look for similar signals, and potentially the opportunity for more robust signals if we have a more potent drug.

[Paul Bolno, President and CEO at Wave Life Sciences]

Also the ability to have a therapy to achieve that, which could potentially be, and as a reminder, we have still the potential for once-a-year dosing. As we think about the opportunity in this field for once or twice-a-year dosing to be able to achieve that alongside, beyond just the treatment of liver fat, all of the other potential cardiometabolic benefits that come with Inhibin E silencing.

[Paul Bolno, President and CEO at Wave Life Sciences]

We think there's a tremendous opportunity in it as a differentiated product for the treatment of MASH, we need to generate that data. I think it's important, as Chris outlined, that the current phase II-A study beyond obesity is designed to elicit these signals so that we can look at the data, evaluate that, and evaluate a potential path forward.

[Paul Bolno, President and CEO at Wave Life Sciences]

As it relates to our fall research day or investor day, as it's evolved to, that's pretty consistent. I think you all have attended. We've done these now for nearly a decade. We try to do that consistent piece of understanding what's coming. If you think about the history over the last couple of years, it was Inhibin E, introduction, and then path to clinic. Last year, PNPLA3, path to clinic.

[Paul Bolno, President and CEO at Wave Life Sciences]

I think there's an opportunity to learn about future programs, I think, as we talked about, bispecifics, an important field that we're opening up, the opportunity to continue to see more there. We'll always guide if that's the best place where data's coming or where updates are coming. As you also know historically, if there's a meaningful update, we'll provide that absent having an event to hold that for. Again, it's always been an opportunity for us to share, whether that's data preclinically or clinically. We have that opportunity coming up, and we'll share more on what's going to be that schedule in the very near future.

[Operator]

Our next question comes from Catherine Novak with JonesTrading. Please unmute your line and ask your question.

[Catherine Novak, Analyst at JonesTrading]

Hi. Morning. Thanks for taking my question. I just wanted to drill down on expectations for monotherapy body weight loss for WVE-007 in patients with type 2 diabetes. We've seen combination data with incretins showing INHBE knockdown might help sensitize patients to therapy. What do you exactly expect to see with monotherapy in type 2 diabetes relative to what you've seen in healthy overweight patients? Thanks.

[Paul Bolno, President and CEO at Wave Life Sciences]

Yeah. No, thank you for the question, because I think early on, there were a lot of discussions that I think confounded this field into diabetics, non-diabetics, and that would be different around thinking about combinations. I think it's important as we think about how we bifurcated it, and again, as we shared on the prior question, the work that's encouraging is that there doesn't appear to be for INHBE and BMI and insulin sensitivity and truncal fat.

[Paul Bolno, President and CEO at Wave Life Sciences]

That observational study that was run was in non-diabetics. This correlation is not tied to a diabetic sub-cohort or population. It's the whole population that would be amenable to that. When we step back and look at it, we're not going to miss an opportunity if there's a signal either in one versus the other, we'll have both.

[Paul Bolno, President and CEO at Wave Life Sciences]

The real opportunity we have in bifurcating and studying the diabetic population is because we have hemoglobin A1C, we'll be able to look at what's seen in human genetics of the ability of an Inhibin E or Activin E reduction to actually improve insulin sensitivity and improve hemoglobin A1C levels potentially. We'll be able to see that signal. The study, as we said, is designed to capture body weight reduction in a patient population that has higher BMI, higher subcute fat, higher visceral fat, look for those cardiometabolic improvements more broadly. Again, not tied specifically to a subpopulation.

[Paul Bolno, President and CEO at Wave Life Sciences]

I think that was kind of a red herring, like a year ago, that I know spiraled into this being a diabetic subpopulation for a fact, actually the data in human genetics and in the population studies that have been run don't demonstrate that to be an effect, we'll have the study to elucidate that.

[Operator]

Our next question comes from Ben Burnett with Wells Fargo. Please unmute your line and ask your question.

[Ben Burnett, Analyst at Wells Fargo]

Great. Thanks so much. I wanted to ask about the 008 program as this is now moving towards the clinic, the PNPLA3 liver disease program. I think you've mentioned that a 50% editing, you would expect to see sort of a positive clinical effect. How did you arrive at that threshold, and is that the threshold that you might expect in patients?

[Paul Bolno, President and CEO at Wave Life Sciences]

Erik, do you want to speak to the genetics and the driver?

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

Yeah. Thanks for the question. This is anchored on the human genetics observations, where it's known that individuals that are homozygous for this variant, they have a nine times higher risk of dying from liver disease during follow-up versus the heterozygous that are just mildly increased risk. The risk reduction, if you go from homozygous state to heterozygous state, is more than 80%. That's kind of what drives this 50%. That's the bar that we want to try to achieve. Getting higher than 50% is also better, but at 50% we would expect to have this therapeutic effect. That's kind of at the center of what.

[Paul Bolno, President and CEO at Wave Life Sciences]

Just to follow onto that, because I think, as we spend more time on this program, because I do think it's an exciting one. I think the confounding data too that's coming out on the siRNA silencing of that enzyme is equivalently important, right? We're talking about now fixing it and repairing it and bringing it up to 50%. I think the human experiments that have shown that decreasing that continually actually leads to potential worsening in liver disease.

[Paul Bolno, President and CEO at Wave Life Sciences]

We have to think about this not just in the fat accumulation, but in the inflammatory aspect and fibrotic aspect, which is actually this particular enzyme works on both, where the fat's located and on that anti-inflammatory, anti-fibrotic effect. I think on both sides, the data continues on human genetics, why 50%, but also why silencing it is potentially detrimental.

[Operator]

Next question comes from Cha Cha Yang with Jefferies. Please unmute your line and ask a question.

[Cha Cha Yang, Analyst at Jefferies]

Hi, this is Cha Cha on for Roger. Thanks for taking our question. Thanks for the updates here. Mostly just a question on timing for your phase II. Can you just let us know what we can expect for your data releases? Are you still going to be doing updates for three month, six month, nine month, et cetera? And then just a sense on timing for that. Thanks.

[Paul Bolno, President and CEO at Wave Life Sciences]

Thanks. As we've said previously, the study's initiating. Recruitment's going extraordinarily well. Dosing's moving. Until once we have a cadence for when we can expect, so we don't have to provide multiple updates on guidance, we'll have a good sense of patients and timing, and we'll provide that updated guidance as soon as we have a sense of the enrollment. I don't know, Chris, is there anything you want to add to that?

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

No. I think you covered it. We're just bringing on some new sites and just trying to accelerate it as much as possible, and there's a lot of interest. Once that's kind of at a steady state, we'll be able to provide guidance.

[Operator]

Our next question comes from Daniel Green with Truist Securities. Please unmute your line and ask your question.

[Alex Nackenoff, Analyst at Truist Securities]

Hey, guys, this is Alex on for Daniel. Thanks for the question. Question on AATD. I know it's a little bit early talking about the potential commercial question, but as we think about the opportunity, any initial market research or anecdotes about physician or patient preference for a mechanism that is not a gene therapy, whether that's hesitation or awareness of off-target permanent editing potential of gene therapeutics. Just curious what your current temperature checks are from patients and clinicians. Thanks so much.

[Paul Bolno, President and CEO at Wave Life Sciences]

Thank you, Alex. Yes, we're doing our market research analysis obviously in preparation for feedback from the agency on potential pathways to accelerated approval. They include not just as you outlined, patients and clinicians, but also payers. I think as we're engaging around this, I think we're seeing a lot of support across all three constituencies.

[Paul Bolno, President and CEO at Wave Life Sciences]

To your point, hesitancy on permanent genetic mutations with the potential for editing cancer-associated genes and what that risk factor looks like if you discharge over time, the risk that maybe you might not be able to be amenable to other editing therapies if we were to come on, not to mention, as you point out, the potential safety risk.

[Paul Bolno, President and CEO at Wave Life Sciences]

As we're seeing that, we're seeing a lot of support for RNA medicines and not dissimilar to other therapeutic spaces like TTR, where people are seeing if you can get through a redosable durable, pushing to potential monthly dosing or less frequent, that ability to see that play out with correction. We're seeing a lot of support for that.

[Paul Bolno, President and CEO at Wave Life Sciences]

As we think about the payer conversations, I think the idea that you need over a decade of safe durability to break even on an RNA medicine, we're seeing a lot of support on that side. Again, as we're testing the constituencies, we're continuing to see a lot of support for RNA medicine approach to AATD.

[Operator]

Our next question comes from Ananda Ghosh with H.C. Wainwright & Co. Please unmute your line and ask your question.

[Ananda Ghosh, Analyst at H.C. Wainwright & Co.]

Hi. Thanks, guys. Paul, continuing on the diabetic question. Historically, the impact of incretins on the body weight in T2D patients has been always lower compared to pure obese patients. Given the differentiated MOA of incretins, what are your thoughts? What do you think the WVE-007 might have an impact on in the diabetic patients differentially? Then I have two follow-up questions.

[Paul Bolno, President and CEO at Wave Life Sciences]

Yeah. Erik, I'll let you start, and then I'll join.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

I think that's a great question, and it's actually at the center of why we think it's important to look at diabetics and non-diabetics separately, because we know that weight loss mechanisms are different mechanistically. For a mechanism like this that is directly driving weight loss through fat loss, we do think that it has a lot of potential for diabetics. To Paul's point, we also know, based on both human genetics and observational studies, that this effect is also important in non-diabetics. That's why we're going to study both in the phase II-A trial.

[Paul Bolno, President and CEO at Wave Life Sciences]

I don't have anything to add to that.

[Operator]

Our next question comes from Cassie Yuan with RBC Capital Markets. Please unmute your line and ask your question.

[Cassie Yuan, Analyst at RBC Capital Markets]

Great. Good morning, guys. Thanks so much for taking our question and squeezing me in. Hi, Paul, and maybe Chris. On your PNPLA3 program, you've elaborated how RNA editing of I148M variant is expected to be superior to siRNA silencing. Meanwhile, Madrigal recently in licensed an siRNA.

[Cassie Yuan, Analyst at RBC Capital Markets]

You did mention preclinical data showing AIMer reduced lipid accumulation more effectively than siRNA, and given evidence that PNPLA3 silencing may also leave residual pathology in inflammation and fibrosis. Just curious how you would articulate a potential clinical differentiation of 008 to patients and potential partners, and how you're thinking about patient enrichment and selection for the first-in-human study. Thanks so much.

[Paul Bolno, President and CEO at Wave Life Sciences]

Thank you. One, I think there's a lot to unpack there. I think on one hand, as you point out, it is a commonly detected mutation. When we think about the ability for PNPLA3, it's in consumer genetic tests.

[Paul Bolno, President and CEO at Wave Life Sciences]

We had a patient here, even just a week ago, discussing the disease from one of the liver foundations, and she found out she had PNPLA3 liver disease through one of her consumer genetic tests. In her charting the diagnosis, she knew she had liver disease and was getting evaluated. I think the drive to actually drive genetic testing there is increasing, so I think that's encouraging. There's work that we're doing, obviously, in preparation for the clinic about enriching and identifying these patients in advance.

[Paul Bolno, President and CEO at Wave Life Sciences]

Again, work that can be done that Chris and the development team are doing to enhance the enrollment and speed with which we can generate human data sets. I think to your point, the growing body of evidence, both in preclinical and clinical data on silencing versus editing, is continuing to accumulate.

[Paul Bolno, President and CEO at Wave Life Sciences]

As we shared at our investor day last year, where we provided some of the preclinical data is, yeah, we see that there's this shift in terms of silencing where we can do siRNA, so we can knock it out if we wanted to. Editing was much more efficient in terms of driving not just fat reduction, but also improvements in other inflammatory biomarkers, and I think there'll be more we can end up sharing there as we think ahead.

[Paul Bolno, President and CEO at Wave Life Sciences]

I think what's encouraging as well, as we think about this distinction, is recent presentations at EASL on siRNA therapies that did show dose-dependent worsening in the liver disease. I think the clinical data is continuing to accumulate on what happens if you knock out that important enzyme, and the data is continuing to obviously amass preclinically.

[Paul Bolno, President and CEO at Wave Life Sciences]

With us going into the clinic and being first, we'll demonstrate human data, the ability to show that by restoring the function of this enzyme, as the human genetics suggests, we should be able to make efficiently not just a treatment for what happens with fat. The reason this enzyme may be at the center of so many different liver diseases beyond just MASH is a function of its impact on this reactive oxygen system, this pro-inflammatory system.

[Paul Bolno, President and CEO at Wave Life Sciences]

It's been seen time and time again that if you can increase this, you can improve the fibrotic side. I don't know, Erik, if there's anything to add on the preclinical data.

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

No, I think you were very comprehensive. The bottom line is that you want to restore it to a function. It has an important wild-type function, so if you knock it out, you can exacerbate some aspects of the disease. That's really where we, by using an RNA editing approach, we're restoring it back to normal.

[Paul Bolno, President and CEO at Wave Life Sciences]

Yeah.

[Operator]

Our last question comes from Mara Goldstein with Clear Street. Please unmute your line and ask your question.

[Mara Goldstein, Analyst at Clear Street]

Great. Thanks so much for taking my question. I know it's late in the call, but I'm just curious if you can provide some color as you're thinking about the cardiometabolic signals that you're looking for WVE-007 in the trial. How do you weight that or rank order what you'd be looking at and what the threshold might be?

[Paul Bolno, President and CEO at Wave Life Sciences]

Thanks for your question. I'll step back from rank ordering. I think they're all important as we think about cardiometabolic diseases. They're all different, right? As we elucidated, the ability, and I think this all stems as Erik shared during the remarks earlier, we think about what's so important ultimately in this disease process is visceral fat.

[Paul Bolno, President and CEO at Wave Life Sciences]

As you go 5%-10%, that difference, that increase in visceral fat, just 5%-10%, drives a whole bunch of different cardiometabolic outputs. This ability to see that substantial reduction in the output of that becomes important as we think of improvement. As we said, we've already seen 14% reduction, 2% reduction in visceral fat. We're consequentially changing that now in an otherwise healthy population. Again, being able to evaluate that in the phase IIa population is interesting for us, right?

[Paul Bolno, President and CEO at Wave Life Sciences]

It's going to allow us to look at each of these markers. What happens with hemoglobin A1c as a function, not just in stratifying patients, diabetics, non, but really the impact on hemoglobin A1c, which by itself is a marker for registration in type 2 diabetes. What's happening with lipid levels and improvement in lipid levels as seen in the human genetics, but also potentially for insulin sensitivity as we think about HDL/triglyceride ratios.

[Paul Bolno, President and CEO at Wave Life Sciences]

The ability to look at that in its compendium of obviously fat loss and weight loss and obesity more general, and MASH with liver fat. I think we're looking at all of these independently and in their global association with each other in the context of obesity and cardiometabolic disease. I don't know, Chris or Erik, if there's anything to add to that.

[Chris Wright, Chief Medical Officer at Wave Life Sciences]

No, I would just say, we'll look at the data and see where we're best differentiated and where we can move forward the fastest. You can think about MASH, for example, where you can get an accelerated approval that could be attractive if we're seeing strong effects on lipids there in the liver. That's sort of how we're looking at it, and just following the data and taking the best things forward into the most accelerated paths we can to get things approved. Yeah.

[Paul Bolno, President and CEO at Wave Life Sciences]

Erik, anything to add?

[Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences]

No, I think you covered it. I guess the only thing I would add is that it all comes back to the mechanism as in driving fat loss and decreasing visceral fat, and that mechanism is really linked to all of these outcomes. There are a lot of opportunities here.

[Paul Bolno, President and CEO at Wave Life Sciences]

Great.

[Operator]

Thank you. There are no further questions at this time. I'll now hand the call back over to Paul Bolno for closing remarks.

[Paul Bolno, President and CEO at Wave Life Sciences]

Thank you for joining our call this morning. We appreciate your continued support. Have a great day.

[Operator]

This concludes today's call. Thank you, everyone, for joining. You may now disconnect.

Participants

Executives

• Paul Bolno, President and CEO
• Kyle Moran, CFO

Analysts

• Kate Rausch, VP of Corporate Affairs and Investor Relations at Wave Life Sciences
• Erik Ingelsson, Chief Scientific Officer at Wave Life Sciences
• Chris Wright, Chief Medical Officer at Wave Life Sciences
• Yun Zhong, Analyst at Wedbush
• Ben Asuncion, Analyst at Citi
• Nick Econom, Analyst at Cantor Fitzgerald
• Joseph Schwartz, Analyst at Leerink Partners
• Angela Qian, Analyst at Canaccord Genuity
• Salim Syed, Analyst at Mizuho
• Alex Stranahan, Analyst at Bank of America
• Madison El-Saadi, Analyst at B. Riley
• Catherine Novak, Analyst at JonesTrading
• Ben Burnett, Analyst at Wells Fargo
• Cha Cha Yang, Analyst at Jefferies
• Alex Nackenoff, Analyst at Truist Securities
• Ananda Ghosh, Analyst at H.C. Wainwright & Co.
• Cassie Yuan, Analyst at RBC Capital Markets
• Mara Goldstein, Analyst at Clear Street